Part:BBa_K3172009:Design

Cecropin B Generator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We sought to streamline the workflow associated with this chassis. As such we designed the part with forward and reverse primers to allow for amplification without the need for cloning and transformation. The DNA could be kept in this double stranded linear form and be used with the cell free kit.

Source

Cecropin B comes from the giant silk moth: Hyalophora cecropia